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| Mizuno,Cassia Suemi; Ampomaah,Winnifred; Mendonça,Fernanda Ribeiro; Andrade,Gabriela Carvalho; Silva,Ariel Maria Nazaré da; Goulart,Mirian Oliveira; Santos,Raquel Alves dos. |
| Abstract The cytotoxicity and genotoxicity of the stilbenes (E)-methyl-4-(3-5-dimethoxystyryl)benzoate (ester), (E)-4-(3-5-dimethoxystyryl)aniline (amino), (Z)-1,3-dimethoxy-5-(4-methoxystyryl)benzene (cis-TMS) and (E)-1,3-dimethoxy-5-(4-methoxystyryl)benzene (trans-TMS) were investigated in this work. Structural modifications of resveratrol, a naturally occurring stilbene, have been previously performed, including the replacement of hydroxyl by different functional groups. Such modifications resulted in significant improvement of target-specific effects on cell death and antiproliferative responses. The parameters were evaluated using XTT assay, clonogenic survival assay and the cytokinesis-block micronucleus assay in CHO-K1 and HepG2 cell lines. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stilbene; Cytotoxicity; Genotoxicity; Micronucleus assay. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400656 |
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| Le Bihanic, Florane; Clerandeau, Christelle; Le Menach, Karyn; Morin, Benedicte; Budzinski, Helene; Cousin, Xavier; Cachot, Jerome. |
| In aquatic environments, polycyclic aromatic hydrocarbons (PAHs) mostly occur as complex mixtures, for which risk assessment remains problematic. To better understand the effects of PAH mixture toxicity on fish early life stages, this study compared the developmental toxicity of three PAH complex mixtures. These mixtures were extracted from a PAH-contaminated sediment (Seine estuary, France) and two oils (Arabian Light and Erika). For each fraction, artificial sediment was spiked at three different environmental concentrations roughly equivalent to 0.5, 4, and 10 μg total PAH g−1 dw. Japanese medaka embryos were incubated on these PAH-spiked sediments throughout their development, right up until hatching. Several endpoints were recorded at different... |
| Tipo: Text |
Palavras-chave: Developmental abnormalities; Comet assay; Micronucleus assay; Larvae locomotion; Oil extract; Pyrolytic extract; Medaka embryo-larval assay. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00188/29920/28385.pdf |
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| Santos,Raquel A.; Teixeira,Ana Claudia; Mayorano,Monica B.; Carrara,Helio H.A.; Andrade,Jurandyr M.; Takahashi,Catarina S.. |
| Breast cancer (BC) is the most prevalent type worldwide, besides being one of the most common causes of death among women. It has been suggested that sporadic BC is most likely caused by low-penetrance genes, including those involved in DNA repair mechanisms. Furthermore, the accumulation of DNA damage may contribute to breast carcinogenesis. In the present study, the relationship between two DNA repair genes, viz., XRCC1 (Arg399Gln) and XRCC3 (Thr241Met) polymorphisms, and the levels of chromosome damage detected in 65 untreated BC women and 85 healthy controls, was investigated. Chromosome damage was evaluated through micronucleus assaying, and genotypes determined by PCR-RFLP methodology. The results showed no alteration in the risk of BC and DNA damage... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA repair polymorphisms; Breast cancer; Micronucleus assay. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400007 |
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| Boriollo,Marcelo Fabiano Gomes; Resende,Marielly Reis; Silva,Thaísla Andrielle da; Públio,Juliana Yoshida; Souza,Luiz Silva; Dias,Carlos Tadeu dos Santos; Oliveira,Nelma de Mello Silva; Fiorini,João Evangelista. |
| The aim of this study was to evaluate the mutagenicity (clastogenicity/aneugenicity) of a glycolic extract of Ziziphus joazeiro bark (GEZJ) by the micronucleus assay in mice bone marrow. Antimutagenic activity was also assessed using treatments associated with GEZJ and doxorubicin (DXR). Mice were evaluated 24-48 h after exposure to positive (N-nitroso-N-ethylurea, NEU - 50 mg.kg-1 and DXR - 5 mg.kg-1) and negative (150 mM NaCl) controls, as well as treatment with GEZJ (0.5-2 g.kg-1), GEZJ (2 g.kg-1) + NEU and GEZJ (2 g.kg-1) + DXR. There were no significant differences in the frequencies of micronucleated polychromatic erythrocytes in mice treated with GEJZ and GEJZ + DXR compared to the negative controls, indicating that GEZJ was not mutagenic. Analysis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimutagenicity; Bone marrow; Doxorubicin; Micronucleus assay; Mutagenicity; Zizyphus joazeiro Mart. (raspa-de-Juá). |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300016 |
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| Lourenço,Eloir D.; Amaral,Viviane S. do; Lehmann,Mauricio; Dihl,Rafael R.; Schmitt,Virginia M.; Cunha,Kênya S.; Reguly,Maria L.; Andrade,Heloisa H.R. de. |
| This study evaluated the clastogenic and/or aneugenic potential of three nucleoside reverse transcriptase inhibitors (zidovudine - AZT, lamivudine - 3TC and stavudine - d4T) using the cytokinesis-block micronucleus (CBMN) assay in human lymphocyte cultures. All three inhibitors produced a positive response when tested in binucleated cells. The genotoxicity of AZT and 3TC was restricted to binucleated cells since there was no significant increase in the frequency of micronuclei in mononucleated cells. This finding indicated that AZT and 3TC caused chromosomal breakage and that their genotoxicity was related to a clastogenic action. In addition to the positive response observed with d4T in binucleated cells, this drug also increased the frequency of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lamivudine; Micronucleus assay; Stavudine; Transcriptase inhibitors; Zidovudine. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400024 |
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| Strubbia, Sofia; Lyons, B. P.; Lee, R. J.. |
| Environmental conditions and xenobiotic exposure can be sources of stress to living organisms. Biological markers are measurable indicator of changes which may happen at any biological level and which can be considered an early warning signal of some biological or environmental state or condition. A structured field study was undertaken to investigate the relationship between three biomarker assays and the spatial and temporal variation of each biomarker in samples of Mytilus edulis. The three biomarkers were the neutral red retention assay, micronucleus assay and comet assay, which indicate damage at different cellular/molecular levels. Three sites in Poole Harbour, an area on the South coast of the UK were sampled on six separate occasions at least three... |
| Tipo: Text |
Palavras-chave: Mytilus edulis; Biomarkers; Stress; Neutral red retention; Comet assay; Micronucleus assay; Poole Harbour. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00481/59274/63447.pdf |
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